The Class C CAZ affects non-Euro-6 trucks from 27 February, as well as light goods vehicles, buses and coaches.
Those who own a business and operate LGVs within Sheffield or Rotherham may qualify for a temporary extension until June 5, 2023.
The Clean Air Zone in Sheffield covers the inner ring road and city centre, with clear signage indicating its boundaries and entry points featuring a green cloud Class C symbol.
Individuals entering the zone have the option to make payments up to six days prior, on the day of entry, or up to six days following their journey.
Councillor Mazher Iqbal, co-chair of Sheffield city council’s transport regeneration and climate policy committee, said it had a “critical mission” to reduce exposure to NO2 emissions.
He added: “We are very aware of the challenges the introduction of the Clean Air Zone poses for businesses and vehicle owners and we empathise with those affected.
“We know it may impact some financially, that’s why we worked hard to secure the best financial package we could from the government for those who need support once the changes are live.”